FUW-hTie1-ECD-FLAG
(Plasmid
#119235)
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PurposePlasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTIE1-ECD-FLAG
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SpeciesH. sapiens (human)
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MutationContains extracellular domain (ECD) only
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Entrez GeneTIE1 (a.k.a. JTK14, LMPHM11, TIE)
- Promoter P hUbC
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-hTie1-ECD-FLAG was a gift from Kari Alitalo (Addgene plasmid # 119235 ; http://n2t.net/addgene:119235 ; RRID:Addgene_119235)