FUW-mTie1
(Plasmid
#119234)
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PurposePlasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTie1
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SpeciesM. musculus (mouse)
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Entrez GeneTie1 (a.k.a. D430008P04Rik, TIE, tie-1)
- Promoter P hUbC
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mTie1 carries a G741D difference compared to the NCBI reference NM_011587.2. This difference is carried in numerous strains found in the Ensembl database and is expected to be functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-mTie1 was a gift from Kari Alitalo (Addgene plasmid # 119234 ; http://n2t.net/addgene:119234 ; RRID:Addgene_119234)