psubCAG-WPRE-hVEGF-D-Strept
(Plasmid
#119227)
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PurposePlasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsubCAG-WPRE
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVEGFD
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SpeciesH. sapiens (human)
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Entrez GeneVEGFD (a.k.a. FIGF, VEGF-D)
- Promoter CAG
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Tag
/ Fusion Protein
- Strept (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psubCAG-WPRE-hVEGF-D-Strept was a gift from Kari Alitalo (Addgene plasmid # 119227 ; http://n2t.net/addgene:119227 ; RRID:Addgene_119227)