gamma1-EGFP
(Plasmid
#119224)
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PurposeGABAA receptor expression (gamma 1 subunit with EGFP tag at the C-terminus)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGABRG1
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SpeciesR. norvegicus (rat)
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GenBank IDNM_080586.1
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Entrez GeneGabrg1
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA originally cloned in Peter Seeburg’s lab in Heidelberg
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gamma1-EGFP was a gift from Angel de Blas (Addgene plasmid # 119224 ; http://n2t.net/addgene:119224 ; RRID:Addgene_119224)