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Addgene

delta-EGFP
(Plasmid #119223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GABRD
  • Species
    R. norvegicus (rat)
  • GenBank ID
    NM_017289.1
  • Entrez Gene
    Gabrd (a.k.a. GABAA-RD)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    rat delta was originally from Dr. Robert Macdonald at Vanderbilt.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delta-EGFP was a gift from Angel de Blas (Addgene plasmid # 119223 ; http://n2t.net/addgene:119223 ; RRID:Addgene_119223)

  • For your References section:

    Clustered and non-clustered GABAA receptors in cultured hippocampal neurons. Christie SB, Li RW, Miralles CP, Yang B-, De Blas AL. Mol Cell Neurosci. 2006 Jan;31(1):1-14. doi: 10.1016/j.mcn.2005.08.014. Epub 2005 Sep 21. 10.1016/j.mcn.2005.08.014 PubMed 16181787

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