delta-EGFP
(Plasmid #119223)

• Purpose: GABAA receptor expression (delta subunit with EGFP tag at the C-terminus)
• Depositing Lab: Angel de Blas
• Publication: Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14. doi: 10.1016/j.mcn.2005.08.014. Epub 2005 Sep 21.

Backbone

• Vector backbone: pEGFP N1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRD
• Species: R. norvegicus (rat)
• GenBank ID: NM_017289.1
• Entrez Gene: Gabrd (a.k.a. GABAA-RD)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: rat delta was originally from Dr. Robert Macdonald at Vanderbilt.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry