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Addgene

pBS592 promoterless EGFPcre
(Plasmid #11921)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11921 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-cre
  • Alt name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1800
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBS592 carries the EGFPcre fusion gene in a promoterless vector with several available restriction sites. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the pBS597 with a tet-controlled promoter and the vector pBS598 having constitutive high level expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS592 promoterless EGFPcre was a gift from Brian Sauer (Addgene plasmid # 11921)

  • For your References section:

    GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853
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