pAMP-CY1_NF1 T2 (+minintr, KDR)
(Plasmid #119201)

• Purpose: transfer vector
• Depositing Lab: Helen Morrison
• Publication: Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu.23681.

Backbone

• Vector backbone: pAMP-CY1
• Backbone manufacturer: Modified from pGEX-4T-1
• Backbone size w/o insert (bp): 2203
• Total vector size (bp): 10788
• Vector type: Transfer vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: DH5a can also be used. Terrific Broth medium can be used to improve plasmid yield, but is not required.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NF1 type 2 mini-gene
• Alt name: neurofibromin type 2
• Species: H. sapiens (human)
• Insert Size (bp): 8663
• GenBank ID: NM_001042492.2, NP_001035957.1
• Entrez Gene: NF1 (a.k.a. NFNS, VRNF, WSS)

Resource Information

• Supplemental Documents:
  • pAMP-CY1_NF1 T2 (+minintr, KDR).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD