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Addgene

pBS500 EF1alpha-GFPcre
(Plasmid #11920)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-cre
  • Alt name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1850
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBS500 carries a GFPcre fusion gene under the control of the elongation factor 1-alpha promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. pBS500 expresses well in murine embryonic stem (ES) cells but this is not the case for the plasmid from which it is derived, pBS448.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS500 EF1alpha-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11920 ; http://n2t.net/addgene:11920 ; RRID:Addgene_11920)

  • For your References section:

    Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Gagneten S, Le Y, Miller J, Sauer B. Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31. 10.1093/nar/25.16.3326 PubMed 9241248
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