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LZF97_hSyn-spine-jRGECO1a
(Plasmid #119198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    fsyn
  • Backbone size w/o insert (bp) 8447
  • Total vector size (bp) 11903
  • Modifications to backbone
    Insertion of ZFn (CCR5-L) binding site and Kozak.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L)
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    3456
  • GenBank ID
    NM_013070.1
  • Entrez Gene
    Utrn
  • Promoter Human Synapsin 1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGACCATCTGCGCTG
  • 3′ sequencing primer GCATTAAAGCAGCGTATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZF97_hSyn-spine-jRGECO1a was a gift from Don Arnold (Addgene plasmid # 119198 ; http://n2t.net/addgene:119198 ; RRID:Addgene_119198)

  • For your References section:

    All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE. Nat Methods. 2018 Oct;15(10):823-831. doi: 10.1038/s41592-018-0142-8. Epub 2018 Oct 1. 10.1038/s41592-018-0142-8 PubMed 30275587
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