pcDNA3.1_RapACR_T111C_N248Q-EYFP
(Plasmid
#119197)
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PurposeMammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 6217
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAnion channelrhodopsin RapACR fast mutant T111C_N248Q
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SpeciesRhodomonas salina
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Insert Size (bp)921
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Mutationchanged threonine 111 to cysteine and asparagine 248 to glutamine
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GenBank IDMG831192
- Promoter CMV
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_RapACR_T111C_N248Q-EYFP was a gift from John Spudich (Addgene plasmid # 119197 ; http://n2t.net/addgene:119197 ; RRID:Addgene_119197)
