pJKW1659
(Plasmid #119180)

• Purpose: E.coli expression plasmid for Pm4CL1 and PmCHS
• Depositing Lab: Jing-Ke Weng
• Publication: Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 10.1038/s41477-019-0474-0.

Backbone

• Vector backbone: pJKW1565
• Backbone size w/o insert (bp): 3233
• Total vector size (bp): 6048
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Pm4CL1
• Species: Piper methysticum
• Insert Size (bp): 1638
• GenBank ID: MK058492
• Promoter: pGAP

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCGCTTGACGCTGCGTAAG

Gene/Insert 2

• Gene/Insert name: PmCHS
• Species: Piper methysticum
• Insert Size (bp): 1185
• GenBank ID: MK058495
• Promoter: pGAP

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTTTGAGGAAGGATTTGAG

Resource Information

• Supplemental Documents:
  • pJKW1659_Pm4CL1_PmCHS_pJKW1565.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry