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Addgene

pJKW1548
(Plasmid #119177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p426TEF
  • Backbone size w/o insert (bp) 6352
  • Total vector size (bp) 7543
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PmCHS
  • Species
    Piper methysticum
  • Insert Size (bp)
    1185
  • GenBank ID
    MK058495
  • Promoter TEF1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TACTTCTTGCTCATTAGAAAGA
  • 3′ sequencing primer tcgaggtcgacggtatcgataa
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW1548 was a gift from Jing-Ke Weng (Addgene plasmid # 119177 ; http://n2t.net/addgene:119177 ; RRID:Addgene_119177)

  • For your References section:

    The biosynthetic origin of psychoactive kavalactones in kava. Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK. Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 10.1038/s41477-019-0474-0. 10.1038/s41477-019-0474-0 PubMed 31332312
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