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Addgene

pJKW1270
(Plasmid #119172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119172 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEAQ-HT
  • Backbone size w/o insert (bp) 10003
  • Total vector size (bp) 11491
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PmCYP719A26
  • Alt name
    PmMTS1
  • Species
    Piper methysticum
  • Insert Size (bp)
    1536
  • GenBank ID
    MK058499
  • Promoter CaMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGGTTCTATAAGAAATCTAG
  • 3′ sequencing primer ACACTCTGAGCACAGAAAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW1270 was a gift from Jing-Ke Weng (Addgene plasmid # 119172)

  • For your References section:

    The biosynthetic origin of psychoactive kavalactones in kava. Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK. Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 10.1038/s41477-019-0474-0. 10.1038/s41477-019-0474-0 PubMed 31332312
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