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pCerulean-VSVG
(Plasmid #11913)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VSVG
  • Alt name
    Vesicular stomatitus virus G protein
  • Insert Size (bp)
    1500
  • Mutation
    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M153T, V163A, A206K relative the wild type GFP sequence. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCerulean-VSVG was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11913 ; http://n2t.net/addgene:11913 ; RRID:Addgene_11913)

  • For your References section:

    ER-to-Golgi transport visualized in living cells. Presley JF, Cole NB, Schroer TA, Hirschberg K, Zaal KJ, Lippincott-Schwartz J. Nature. 1997 Sep 4. 389(6646):81-5. 10.1038/38001 PubMed 9288971
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