eGFP L202 Reporter
(Plasmid #119129)

• Purpose: Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.
• Depositing Lab: Reuben Harris
• Publication: Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.

Backbone

• Vector backbone: plenti-CMV-mCherry-T2A-GFP
• Backbone size w/o insert (bp): 9990
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry and eGFP
• Insert Size (bp): 1506
• Mutation: eGFP L202S
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: ctggctaccggtatggtgagcaagggcgagg
• 3′ sequencing primer: CTTGTACTCGAGATCTGCACCGGGCTTGTACAGCTCGTCCATGCC

Resource Information

• Supplemental Documents:
  • eGFP L202 Reporter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry