pRSETA-PAGFP
(Plasmid #11911)

• Depositing Lab: Jennifer Lippincott-Schwartz
• Publication: Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Backbone

• Vector backbone: pRSETA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2827
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pRSETA-PAGFP
• Alt name: photoactivatable green fluorescent protein
• Species: Aequorea victoria
• Insert Size (bp): 720
• Mutation: The PAGFP has four mutations (L64F/T65S/V163A/T203H) compared with the Clontech EGFP. The PAGFP also contains an A206K mutation to disrupt dimerization

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.

The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.