pCUPI NPD-GFP
(Plasmid
#1191)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCLUC
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Backbone manufacturerThiele
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Vector typeYeast Expression ; CUP1 promoter
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN-terminus of Sup35
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Alt nameSup35
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1200
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Mutationfused N-terminal Sup35 (~1-253aa) to GFP
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GenBank IDNC_001136
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Entrez GeneSUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site zGorI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCUPI NPD-GFP was a gift from Susan Lindquist (Addgene plasmid # 1191 ; http://n2t.net/addgene:1191 ; RRID:Addgene_1191) -
For your References section:
Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547