pFGL1010
(Plasmid
#119081)
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Purpose(Empty Backbone) Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFGL1009
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Backbone manufacturerNaweed Lab
- Backbone size (bp) 10110
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Modifications to backboneNcTUB2 terminator was added.
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Vector typeFungal expression (in Magnaporthe oryzae)
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Selectable markersSulfonylurea (Chlorimuron ethyl)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer taaGTCGACtaaatcattccactcaacattcaggct
- 3′ sequencing primer gcCTCGAGcatcatcatgcaacatgcatgtact (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFGL1010 was a gift from Naweed Naqvi (Addgene plasmid # 119081 ; http://n2t.net/addgene:119081 ; RRID:Addgene_119081) -
For your References section:
A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Patkar RN, Benke PI, Qu Z, Chen YY, Yang F, Swarup S, Naqvi NI. Nat Chem Biol. 2015 Sep;11(9):733-40. doi: 10.1038/nchembio.1885. Epub 2015 Aug 10. 10.1038/nchembio.1885 PubMed 26258762
Map uploaded by the depositor.
