mCherry-CEPT1
(Plasmid #119079)

• Purpose: Visualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholine
• Depositing Lab: Gia Voeltz
• Publication: English et al Nat Cell Biol. 2013 Feb;15(2):169-78. doi: 10.1038/ncb2647. Epub 2012 Dec 23.

Backbone

• Vector backbone: mCherry-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5116
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CEPT1
• Species: H. sapiens (human)
• GenBank ID: NM_006090
• Entrez Gene: CEPT1 (a.k.a. MGC45223, DKFZp313G0615)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV Foward
• 3′ sequencing primer: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry