pVC-Ds-DrU6a:sgRNA-MS2-Ds
(Plasmid #119076)

• Purpose: To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops
• Depositing Lab: Tatjana Sauka-Spengler
• Publication: Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. Epub 2023 Jun 27.

Backbone

• Vector backbone: Unknown
• Backbone size w/o insert (bp): 3565
• Total vector size (bp): 4141
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6a:2xMS2-sgRNA
• Species: D. rerio (zebrafish)
• Insert Size (bp): 576
• Promoter: Zebrafish U6a promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: U6a:sgRNA sequence from pU6a:sgRNA#1 (Addgene #64245). MS2 stem loop sequence from Konermann, Brigham et al. 2014.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.