pBac(3xP3-gTc’v; Valcyrie.LR)
(Plasmid
#119066)
-
PurposeValcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 119066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBac[3xP3-gTc'v]
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert namepBacL
Gene/Insert 2
-
Gene/Insert nameTc-U6a-Lox.L
Gene/Insert 3
-
Gene/Insert nameTc-hsp68 Promoter
Gene/Insert 4
-
Gene/Insert nameNLS-Cas9
Gene/Insert 5
-
Gene/Insert nameTc-hsp68 3'UTR
Gene/Insert 6
-
Gene/Insert nameTc-U6B-Lox.R
Gene/Insert 7
-
Gene/Insert nameSV40polyA
Gene/Insert 8
-
Gene/Insert nameTc-vermilion
Gene/Insert 9
-
Gene/Insert name3xP3 TATA
Gene/Insert 10
-
Gene/Insert namepBacR
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/biorxiv/early/2018/08/17/394221.full.pdf for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBac(3xP3-gTc’v; Valcyrie.LR) was a gift from Michalis Averof (Addgene plasmid # 119066 ; http://n2t.net/addgene:119066 ; RRID:Addgene_119066) -
For your References section:
Clonal analysis by tunable CRISPR-mediated excision. Gilles AF, Schinko JB, Schacht MI, Enjolras C, Averof M. Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 10.1242/dev.170969. 10.1242/dev.170969 PubMed 30552128