pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
(Plasmid #119064)

• Purpose: Cre/lox flip-out construct for marking cell clones in Tribolium castaneum
• Depositing Lab: Michalis Averof
• Publication: Gilles et al Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 10.1242/dev.170969.

Backbone

• Vector backbone: pBac[3xP3-gTc'v]
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pBacL
• Insert Size (bp): 1425

Gene/Insert 2

• Gene/Insert name: pUb regulatory sequence
• Insert Size (bp): 941

Gene/Insert 3

• Gene/Insert name: loxN

Gene/Insert 4

• Gene/Insert name: mYFP

Gene/Insert 5

• Gene/Insert name: loxN

Gene/Insert 6

• Gene/Insert name: H2B-mcherry

Gene/Insert 7

• Gene/Insert name: SV40polyA

Gene/Insert 8

• Gene/Insert name: Tc-vermilion

Gene/Insert 9

• Gene/Insert name: 3xP3 TATA

Gene/Insert 10

• Gene/Insert name: pBacR

Resource Information

• Supplemental Documents:
  • pBac(3xP3-gTc’v; pUb_lox-mYFP-lox-H2BmCherry).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/biorxiv/early/2018/08/17/394221.full.pdf for BioRxiv preprint.