pVITRO-Fab_Quin-LUCID
(Plasmid
#119049)
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PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119049 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVITRO1-Hygro
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Backbone manufacturerInvivogen
- Backbone size w/o insert (bp) 6295
- Total vector size (bp) 9225
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameFab_Quin-LUCID_part1
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Alt nameSNAP_p30_nLuc_Fab Quin (VL)
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SpeciesSynthetic
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Insert Size (bp)1950
- Promoter EF-1α + CMV/SV40 enhancers
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Tags
/ Fusion Proteins
- Strep-tag (C terminal on insert)
- His-tag (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer mAB-S3: cagGTGTTGTGAAagCC
- 3′ sequencing primer mAB-S7: CCTTTTTTAGGTGTTGTGAAAaC (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameFab_Quin-LUCID_part2
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Alt nameFab Quin (VL)
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SpeciesSynthetic
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Insert Size (bp)810
- Promoter IRES
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Tag
/ Fusion Protein
- FLAG-tag
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer mAB-S8: gGGATTTTGGTCATGGCTAG
- 3′ sequencing primer mAB-S9: TGGTGTACCCTGTGGATG (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVITRO-Fab_Quin-LUCID was a gift from Kai Johnsson (Addgene plasmid # 119049 ; http://n2t.net/addgene:119049 ; RRID:Addgene_119049) -
For your References section:
Bioluminescent Antibodies for Point-of-Care Diagnostics. Xue L, Yu Q, Griss R, Schena A, Johnsson K. Angew Chem Int Ed Engl. 2017 Jun 12;56(25):7112-7116. doi: 10.1002/anie.201702403. Epub 2017 May 16. 10.1002/anie.201702403 PubMed 28510347