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pBAD-MTX-u23_Snifit
(Plasmid #119044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 5216
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTX-u23_Snifit
  • Alt name
    SNAP_Pro30_DHFRun23 R44L H45Q
  • Species
    Synthetic
  • Insert Size (bp)
    1251
  • Mutation
    23TAG R44L H45Q
  • Promoter pBAD
  • Tags / Fusion Proteins
    • Strep-tag (N terminal on insert)
    • His-tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-MTX-u23_Snifit was a gift from Kai Johnsson (Addgene plasmid # 119044 ; http://n2t.net/addgene:119044 ; RRID:Addgene_119044)

  • For your References section:

    A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range. Xue L, Prifti E, Johnsson K. J Am Chem Soc. 2016 Apr 27;138(16):5258-61. doi: 10.1021/jacs.6b03034. Epub 2016 Apr 15. 10.1021/jacs.6b03034 PubMed 27071001
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