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Addgene

PB-BE3-2NLS
(Plasmid #119031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac-mRFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BE3
  • Insert Size (bp)
    5247
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-BE3-2NLS was a gift from Jinsong Li (Addgene plasmid # 119031 ; http://n2t.net/addgene:119031 ; RRID:Addgene_119031)

  • For your References section:

    CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Li Q, Li Y, Yang S, Huang S, Yan M, Ding Y, Tang W, Lou X, Yin Q, Sun Z, Lu L, Shi H, Wang H, Chen Y, Li J. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. 10.1038/s41556-018-0202-4 [pii] PubMed 30275529
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