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PurposeExpress GFP-Daxx in mamalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedeath-domain associated protein (Daxx)
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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Entrez GeneDAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Daxx/pEGFP was a gift from Xiaolu Yang (Addgene plasmid # 119021 ; http://n2t.net/addgene:119021 ; RRID:Addgene_119021) -
For your References section:
Phosphorylation of Daxx by ATM contributes to DNA damage-induced p53 activation. Tang J, Agrawal T, Cheng Q, Qu L, Brewer MD, Chen J, Yang X. PLoS One. 2013;8(2):e55813. doi: 10.1371/journal.pone.0055813. Epub 2013 Feb 6. 10.1371/journal.pone.0055813 PubMed 23405218