pRVV655
(Plasmid
#119019)
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PurposeDrives TP901-1 recombinase expression in the D. melanogaster germ line
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK+
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 12555
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTP901-1 recombinase
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SpeciesT901-1 Lactococcus phage
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Insert Size (bp)1488
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Tag
/ Fusion Protein
- SV40 NLS (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVV655 was a gift from Richard Mann (Addgene plasmid # 119019 ; http://n2t.net/addgene:119019 ; RRID:Addgene_119019) -
For your References section:
TP901-1 Phage Recombinase Facilitates Genome Engineering in Drosophila melanogaster. Voutev R, Mann RS. G3 (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 10.1534/g3.119.0002. 10.1534/g3.119.0002 PubMed 30696702