pU6Rosa-CAG-Gal4-Rad18UBD
(Plasmid
#119012)
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PurposeMammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 8062
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal4
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneGAL4 (a.k.a. YPL248C, GAL81)
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AsiSI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6Rosa-CAG-Gal4-Rad18UBD was a gift from Ralf Kuehn (Addgene plasmid # 119012 ; http://n2t.net/addgene:119012 ; RRID:Addgene_119012) -
For your References section:
Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination. Bashir S, Dang T, Rossius J, Wolf J, Kuhn R. BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 10.1186/s12896-020-00650-x. 10.1186/s12896-020-00650-x PubMed 33097066