pCAG-TetR-RNF169UBD
(Plasmid #119011)

• Purpose: Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression
• Depositing Lab: Ralf Kuehn
• Publication: Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 10.1186/s12896-020-00650-x.

Backbone

• Vector backbone: Bluescript
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 8549
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetR
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • pCAG-TetR-RNF169UBD.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry