pAAVS1-TLR6
(Plasmid #118997)

• Purpose: Targeting vector for human AAVS1 with TLR6 reporter insert
• Depositing Lab: Ralf Kuehn
• Publication: Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 10.1186/s12896-020-00650-x.

Backbone

• Vector backbone: Bluescript
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 10190
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAVS1
• Species: H. sapiens (human)
• Entrez Gene: PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)
• Entrez Gene: AAVS1 (a.k.a. AAV)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (destroyed during cloning)
• 3′ cloning site: MluI (destroyed during cloning)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • pAAVS1-TLR6.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry