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pFGL1259
(Plasmid #118996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL1010
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 10383
  • Total vector size (bp) 11094
  • Modifications to backbone
    Insertion of eGFP (without start and stop codons)
  • Vector type
    Fungal expression
  • Selectable markers
    Sulfonylurea (Chlorimuron ethyl)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    726
  • Promoter promoter less

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer CATCGGTACCGTGAGCAAGGGCGAGGAGCTGT
  • 3′ sequencing primer CGCGGATCCCTTGTACAGCTCGTCCATGCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1259 was a gift from Naweed Naqvi (Addgene plasmid # 118996 ; http://n2t.net/addgene:118996 ; RRID:Addgene_118996)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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