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Addgene

pFGL1269R
(Plasmid #118994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL815N
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 6845
  • Total vector size (bp) 8096
  • Modifications to backbone
    Insertion of Bar:mCherry (without ATG)
  • Vector type
    Fungal expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bar:mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1266
  • Promoter promoter less

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer CTCggtaccAGCCCAGAACGACGCCCG
  • 3′ sequencing primer CGCGGATCCTTACTTGTACAGCTCGTCCATGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1269R was a gift from Naweed Naqvi (Addgene plasmid # 118994 ; http://n2t.net/addgene:118994 ; RRID:Addgene_118994)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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