pFGL1252_TetGFP(Hyg)
(Plasmid #118993)

• Purpose: TetOFF controlled GFP-tagging vector with Hygromycin selection
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL125_TetOFF(Hyg)
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 10687
• Total vector size (bp): 11398
• Modifications to backbone: Tet OFF cassette fused with eGFP (no stop codon)
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: Synthetic
• Insert Size (bp): 723
• Promoter: Ustilago maydis Mfa1 basal promoter with six tetO binding sites

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (destroyed during cloning)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: GGTCATATGGTGAGCAAGGGCGAGGAG
• 3′ sequencing primer: TAGGGTACCCTTGTACAGCTCGTCCATGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry