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pFGL1252_TetOFF(Hyg)
(Plasmid #118992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL1252
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 6845
  • Total vector size (bp) 10687
  • Vector type
    Fungal expression (in Magnaporthe oryzae)
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tTA+Tnos
  • Species
    Synthetic
  • Insert Size (bp)
    1182
  • Mutation
    Codon optimized for Magnaporthe oryzae
  • Promoter promoter less

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer CGCGAATTCATGGCCCGCCTGGATAAGTCAAAG
  • 3′ sequencing primer CAGGTCGACGCGGCCGCAATTCTCATGTTTGACA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Hygromycin cassette
  • Alt name
    hptII
  • Species
    E. coli
  • Insert Size (bp)
    2008
  • Promoter Aspergillus nidulans TrpC promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer CCGTCTAGAGTCGAGGATATTGAAGGAGCACT
  • 3′ sequencing primer CGCGTCGACAAGAAGGATTACCTCTAAAC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Ttub+PtetO
  • Species
    Synthetic
  • Insert Size (bp)
    682
  • Promoter Ustilago maydis Mfa1 basal promoter with six tetO binding sites

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer GACTCTAGATAAATCATTCCACTCAACATTCAG
  • 3′ sequencing primer CCCGGTACCCATATTAATTGATAGAAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1252_TetOFF(Hyg) was a gift from Naweed Naqvi (Addgene plasmid # 118992 ; http://n2t.net/addgene:118992 ; RRID:Addgene_118992)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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