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pC-KIT5
(Plasmid #118987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHEK2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5589
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-kit proto-oncogene {promoter}
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1215
  • Mutation
    mutK2-mutSP-K1
  • GenBank ID
    S67773.1
  • Entrez Gene
    KIT (a.k.a. C-Kit, CD117, MASTC, PBT, SCFR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC-KIT5 was a gift from Bjorn Hogberg (Addgene plasmid # 118987 ; http://n2t.net/addgene:118987 ; RRID:Addgene_118987)

  • For your References section:

    Interplay of Three G-Quadruplex Units in the KIT Promoter. Ducani C, Bernardinelli G, Hogberg B, Keppler BK, Terenzi A. J Am Chem Soc. 2019 Jul 3;141(26):10205-10213. doi: 10.1021/jacs.8b12753. Epub 2019 Jun 20. 10.1021/jacs.8b12753 PubMed 31244182
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