pC-KIT2
(Plasmid #118984)

• Purpose: Mutational dissection of the G rich region of the c-kit promoter
• Depositing Lab: Bjorn Hogberg
• Publication: Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-10213. doi: 10.1021/jacs.8b12753. Epub 2019 Jun 20.

Backbone

• Vector backbone: psiCHEK2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5589
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-kit proto-oncogene {promoter}
• Species: H. sapiens (human)
• Insert Size (bp): 1215
• Mutation: mutK2-SP-K1
• GenBank ID: S67773.1
• Entrez Gene: KIT (a.k.a. C-Kit, CD117, MASTC, PBT, SCFR)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry