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Addgene

Ptch1-mCherry
(Plasmid #118982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry2-N1
  • Backbone size w/o insert (bp) 4723
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ptch1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4473
  • Mutation
    see depositor comments below
  • Entrez Gene
    Ptch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site XmaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation F600L in mouse Ptch1 was found during Addgene's quality control process. The depositing lab noted this mutation should not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ptch1-mCherry was a gift from Paul Hamel (Addgene plasmid # 118982 ; http://n2t.net/addgene:118982 ; RRID:Addgene_118982)
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