mRuby2-N1 3xFLAG
(Plasmid
#118980)
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Purpose(Empty Backbone) Red fluorescent labelling of proteins combined with FLAG epitope tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemRuby2-N1
- Backbone size (bp) 4803
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- mRuby-FLAG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' site: BsrGI (not destroyed), 3' site: BsrGI (destroyed)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRuby2-N1 3xFLAG was a gift from Paul Hamel (Addgene plasmid # 118980 ; http://n2t.net/addgene:118980 ; RRID:Addgene_118980)
