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puc19-pCas13a
(Plasmid #118963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PUC19MinusMCS
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas13a
  • Alt name
    Plant codon-optimized C2c2
  • Species
    A. thaliana (mustard weed)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    puc19-pCas13a was a gift from Magdy Mahfouz (Addgene plasmid # 118963 ; http://n2t.net/addgene:118963 ; RRID:Addgene_118963)

  • For your References section:

    RNA virus interference via CRISPR/Cas13a system in plants. Aman R, Ali Z, Butt H, Mahas A, Aljedaani F, Khan MZ, Ding S, Mahfouz M. Genome Biol. 2018 Jan 4;19(1):1. doi: 10.1186/s13059-017-1381-1. 10.1186/s13059-017-1381-1 PubMed 29301551
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