alpha5-EGFP
(Plasmid #118956)

• Purpose: GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.
• Depositing Lab: Angel de Blas
• Publication: GABAA receptor expression (unpublished)

Backbone

• Vector backbone: pEGFP N1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRA5
• Species: R. norvegicus (rat)
• Entrez Gene: Gabra5
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: Kpn I (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was originally cloned in Peter Seeburg’s lab in Heidelberg.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry