iRFP682-Smad2
(Plasmid #118943)

• Purpose: Mammalian expression of human Smad2 protein
• Depositing Lab: Won Do Heo
• Publication: Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 10.1021/acssynbio.7b00225. Epub 2018 Jan 12.

Backbone

• Vector backbone: piRFP682-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: iRFP682 gene was removed and re-inserted at restriction enzyme sites NheI and BsrGI.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: iRFP682-Smad2
• Species: H. sapiens (human)
• Insert Size (bp): 1404
• Entrez Gene: SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • iRFP682 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pAR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry