pCMV-OptoTGFBRs
(Plasmid #118942)

• Purpose: Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)
• Depositing Lab: Won Do Heo
• Publication: Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 10.1021/acssynbio.7b00225. Epub 2018 Jan 12.

Backbone

• Vector backbone: ptdToamto-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3925
• Total vector size (bp): 9821
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OptoTGFBRs
• Alt name: Lyn-cytoTGFbR1-CIBN-P2A-cytoTGFbR2-PHR-tdTomato
• Species: M. musculus (mouse), A. thaliana (mustard weed)
• GenBank ID: NM_009370 NM_029575
• Entrez Gene: Tgfb1 (a.k.a. TGF-beta1, TGFbeta1, Tgfb, Tgfb-1)
• Entrez Gene: Tgfbr2 (a.k.a. 1110020H15Rik, DNIIR, RIIDN, TBR-II, TbetaR-II, TbetaRII)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pAR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry