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Addgene

pSOMA-T679A-NES
(Plasmid #118940)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRI 201-AN
  • Vector type
    Plant Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
  • Alt name
    SOMA-T679A-NES
  • Species
    A. thaliana (mustard weed), Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSOMA-T679A-NES was a gift from Patrick Krysan (Addgene plasmid # 118940 ; http://n2t.net/addgene:118940 ; RRID:Addgene_118940)

  • For your References section:

    A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis. Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ. Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. 10.1111/tpj.14164 PubMed 30444549
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