pSOMA-T679A-NLS
(Plasmid #118938)

• Purpose: Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
• Depositing Lab: Patrick Krysan
• Publication: Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.

Backbone

• Vector backbone: pCAMBIA-1300
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
• Alt name: SOMA-T679A-NLS
• Species: A. thaliana (mustard weed), Synthetic

Resource Information

• Supplemental Documents:
  • pSOMA-T679A-NLS.gb
  • pSOMA-T679A-NLS SnapGene File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.