pSOMA-NLS
(Plasmid
#118937)
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PurposeUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAMBIA-1300
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
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Alt nameSOMA-NLS
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SpeciesA. thaliana (mustard weed), Synthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSOMA-NLS was a gift from Patrick Krysan (Addgene plasmid # 118937 ; http://n2t.net/addgene:118937 ; RRID:Addgene_118937) -
For your References section:
A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis. Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ. Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. 10.1111/tpj.14164 PubMed 30444549