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Addgene

pET-SOMA-T679A
(Plasmid #118936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-32(a)+
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
  • Alt name
    SOMA FRET Sensor T679A
  • Species
    A. thaliana (mustard weed), Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-SOMA-T679A was a gift from Patrick Krysan (Addgene plasmid # 118936 ; http://n2t.net/addgene:118936 ; RRID:Addgene_118936)

  • For your References section:

    A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis. Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ. Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. 10.1111/tpj.14164 PubMed 30444549
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