pOCC218
(Plasmid
#118907)
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Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOET1
- Backbone size w/o insert (bp) 4900
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
- Promoter polH
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Tag
/ Fusion Protein
- HIS10, cleavable with 3C protease, YBBR tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOCC218 was a gift from Marc Bickle & PEPC MPI CBG (Addgene plasmid # 118907 ; http://n2t.net/addgene:118907 ; RRID:Addgene_118907) -
For your References section:
FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing. Lemaitre RP, Bogdanova A, Borgonovo B, Woodruff JB, Drechsel DN. BMC Biotechnol. 2019 Mar 29;19(1):20. doi: 10.1186/s12896-019-0512-z. 10.1186/s12896-019-0512-z PubMed 30925874
