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Addgene

pOCC139
(Plasmid #118872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOET1
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
  • Promoter polH
  • Tags / Fusion Proteins
    • HIS6, cleavable with 3C protease (N terminal on backbone)
    • mGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOCC139 was a gift from Marc Bickle & PEPC MPI CBG (Addgene plasmid # 118872 ; http://n2t.net/addgene:118872 ; RRID:Addgene_118872)

  • For your References section:

    FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing. Lemaitre RP, Bogdanova A, Borgonovo B, Woodruff JB, Drechsel DN. BMC Biotechnol. 2019 Mar 29;19(1):20. doi: 10.1186/s12896-019-0512-z. 10.1186/s12896-019-0512-z PubMed 30925874
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