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Addgene

pQLinkHD_EBFP2-LSSmOrange
(Plasmid #118856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQLinkHD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EBFP2-LSSmOrange
  • Mutation
    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOrangeA44V,F84L, W145M, I165D, M167L, G202D substitutions and and TGAGGGGLG between FPs
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pQE_F CCCGAAAAGTGCCACCTG or pQE_65 TGAGCGGATAACAATTTCACACAG
  • 3′ sequencing primer pQE_R GTTCTGAGGTCATTACTGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQLinkHD_EBFP2-LSSmOrange was a gift from Marc Birtwistle (Addgene plasmid # 118856 ; http://n2t.net/addgene:118856 ; RRID:Addgene_118856)

  • For your References section:

    Fluorescence Multiplexing with Spectral Imaging and Combinatorics. Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, Birtwistle MR. ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 10.1021/acscombsci.8b00101. Epub 2018 Oct 30. 10.1021/acscombsci.8b00101 PubMed 30339749
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