YFP-DHFR-myc
(Plasmid
#118852)
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Purpose(Empty Backbone) For fluorophore translocation assay and inducible tagging with zapalog.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Zeo(+)
- Backbone size (bp) 6189
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersZeocin
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Tags
/ Fusion Proteins
- YFP (N terminal on backbone)
- DHFR (C terminal on backbone)
- Myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-DHFR-myc was a gift from Thomas Schwarz (Addgene plasmid # 118852 ; http://n2t.net/addgene:118852 ; RRID:Addgene_118852) -
For your References section:
The light-sensitive dimerizer zapalog reveals distinct modes of immobilization for axonal mitochondria. Gutnick A, Banghart MR, West ER, Schwarz TL. Nat Cell Biol. 2019 Jun;21(6):768-777. doi: 10.1038/s41556-019-0317-2. Epub 2019 May 6. 10.1038/s41556-019-0317-2 PubMed 31061466
